Portions of the following information were taken from Florida Geological Survey Bulletin 66.

The spring is located 4.5 miles north of Fanning Springs on the east side of the Suwannee River. After crossing over the Suwannee River on US 98/27A heading east, turn north (left) on SR 26 and travel north on SR 26 approximately 1.4 miles to the town of Wilcox. In Wilcox, SR 26 makes a 90 degree bend to the east (right). At this bend continue north (straight) onto CR 232. Once on CR 232 continue north to the intersection with CR 334 approximately 1.7 miles. Turn west (left) onto CR 334 and drive approximately 2.3 miles to the boat ramp. The spring run enters the river 0.5 miles upstream from the CR 334 boat ramp.

Description - Otter Spring has a nearly circular, bowl-shaped spring pool measuring 68 ft in diameter. It is surrounded by a concrete bag retaining wall. The spring issues from a vertical fissure in limestone. The depth over the fissure measures 27.5 ft. There was a very slight boil on the pool surface in April 2002. The water is clear and greenish. There is very little aquatic vegetation within the spring; however, algae cover the entire spring depression. A small, limestone, man-made dam stretches across the outflow channel on the west side of the pool. A distinct hydrogen sulfide odor was present at the spring in April 2002. The flow is southwest approximately 110 ft into a larger, apparently man-made, circular pool having a diameter of about 130 ft. The larger pool averages 10 ft deep and is utilized as a swimming area. Otter Spring discharges westward 0.8 miles where it joins the Suwannee River. Its shallow, sand bottomed run is approximately half as wide as the spring. The run flows through the heavily forested river floodplain. Land surrounding the spring is relatively low and rises to approximately 5 ft above water level. Most of the uplands are covered in large grassy areas with interspersed live oak trees. Private residences are visible from the spring several hundred feet (90 plus meters) to the east through oak trees.

Utilization - The spring is surrounded by private property and was previously operated as a private campground and swimming area.